Simple, accurate and economic method of HPTLC has been described for the simultaneous estimation of abacavir sulfate and lamivudine from tablet dosage form. Abacavir sulfate shows absorption maximum at 284.0 nm and lamivudine shows absorption maximum at 270.0 nm in distilled water. Beers law was obeyed in the concentration range of 100-700 ng/spot for both abacavir sulfate and lamivudine in tablet dosage form. The coefficient correlations were found to be 0.997 for LAM and 0.9979 for ABAC respectively in tablet dosage form. Analytical methods employed for quantitative determination of drugs are the key determinants in generating reproducible and reliable data that in turn are used in the evaluation and interpretation of bioavailability, bioequivalence and pharmacokinetics. Absolute recovery of abacavir sulfate ranged from 99.70% while lamivudine recoveries found to be 100.23%.
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